Archives for September 2015

Non-Protein Coding RNA Genes as the Novel Diagnostic Markers for the Discrimination of Salmonella Species Using PCR

September 30, 2015 Oleh Editor

Abstract – Salmonellosis, a communicable disease caused by members of the Salmonella species, transmitted to humans through contaminated food or water. It is of paramount importance, to generate accurate detection methods for discriminating the various Salmonella species that cause severe infection in humans, including S. Typhi and S. Paratyphi A. Here, we formulated a strategy of detection and differentiation of salmonellosis by a multiplex polymerase chain reaction assay using S. Typhi non-protein coding RNA (sRNA) genes. With the designed sequences that specifically detect sRNA genes from S. Typhi and S. Paratyphi A, a detection limit of up to 10 pg was achieved. Moreover, in a stool-seeding experiment with S. Typhi and S. Paratyphi A, we have attained a respective detection limit of 15 and 1.5 CFU/mL. The designed strategy using sRNA genes shown here is comparatively sensitive and specific, suitable for clinical diagnosis and disease surveillance, and sRNAs represent an excellent molecular target for infectious disease.

Corresponding Author: Subash C B Gopinath
Corresponding Author’s Email: subash@unimap.edu.my

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Monitoring recombinant human erythropoietin abuse among athletes

September 30, 2015 Oleh Editor

Abstract – The illegal administration of recombinant human erythropoietin (rHuEPO) among athletes is largely preferred over blood doping to enhance stamina. The advent of recombinant DNA technology allowed the expression of EPO-encoding genes in several eukaryotic hosts to produce rHuEPO, and today these performance-enhancing drugs are readily available. As a mimetic of endogenous EPO (eEPO), rHuEPO augments the oxygen carrying capacity of blood. Thus, monitoring the illicit use of rHuEPO among athletes is crucial in ensuring an even playing field and maintaining the welfare of athletes. A number of rHuEPO detection methods currently exist, including measurement of hematologic parameters, gene-based detection methods, glycomics, use of peptide markers, electrophoresis, isoelectric focusing (IEF)-double immunoblotting, aptamer/antibody-based methods, and lateral flow tests. This review gleans these different strategies and highlights the leading molecular recognition elements that have potential roles in rHuEPO doping detection.

Corresponding Author: Subash C B Gopinath
Corresponding Author’s Email: subash@unimap.edu.my

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'Bunga Nano Berbintik': Nanohibrid Zink Oksida Berbiji Emas untuk Bio-tangkapan Selektif

8 September 2015 Oleh Editor

Abstrak – Nanostruktur emas hibrid yang disemai ke dalam bunga nano zink oksida (ZnO) bertekstur nano (NF) telah dicipta untuk aplikasi biosensing baharu. 'NF berbintik' yang dipilih mempunyai lapisan nanopartikel emas (AuNP) setebal 30 nm, dipilih daripada pelbagai ketebalan AuNP, yang disembur ke permukaan. Nanohibrid yang dihasilkan, yang dicirikan oleh analisis morfologi, fizikal dan struktur, telah disemai secara seragam dengan AuNP ke dalam NF ZnO dengan panjang purata 2–3 μm. Penangkapan terpilih prob molekul ke atas AuNP yang disemai adalah bukti untuk interaksi khusus dengan DNA daripada strain penyebab Leptospirosis patogenik melalui analisis hibridisasi dan salah padanan. Had pengesanan yang dicapai ialah 100 fM seperti yang ditentukan melalui spektroskopi impedans. Tahap kestabilan, kebolehulangan dan penjanaan semula sensor yang tinggi telah diperolehi. Imobilisasi dan hibridisasi DNA terpilih telah disahkan oleh puncak nitrogen dan fosforus dalam analisis spektroskopi fotoelektron sinar-X. Hibrid nanostruktur yang dicipta menerangi mekanisme penjanaan pengesanan berprestasi tinggi berbilang sasaran pada platform NF tunggal, yang membuka jalan baharu untuk diagnostik perubatan berasaskan tatasusunan.

Penulis Berkaitan: Uda Hashim
Emel Pengarang Berkaitan: uda@unimap.edu.my

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