A talk on Nano Biosensors Seminar by Prof. Dr. Uda Hashim from Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis (UniMAP).
Venue: Research Frontier Hall, Universiti Teknologi PETRONAS (UTP)
Date: March 4, 2015
A Portable Automatic Endpoint Detection System for Amplicons of Loop Mediated Isothermal Amplification on Microfluidic Compact Disk Platform
Abstract – In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10−3 ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment.
Keywords – pathogen, Salmonella, food safety, diagnosis, LAMP, multiplexed detection, endpoint detection system, microfluidics, compact disc, lab-on-a-CD
Corresponding Author: Uda Hashim
Corresponding Author’s Email: uda@unimap.edu.my
Full text: PDF
Public-Private Research Network (PPRN) 2015
Sekitar majlis pelancaran Public-Private Research Network (PPRN) yang telah disempurnakan oleh YAB Perdana Menteri Malaysia, Dato’ Sri Mohd Najib Bin Tun Abdul Razak pada 24 Februari 2015 bertempat di Dewan Perdana, Putrajaya International Convention Centre (PICC), Putrajaya.
Fabrication of Interdigitated Electrodes (IDE’s) by Conventional Photolithography Technique for pH Measurement Using Micro-Gap Structure
Abstract – Micro interdigitated electrodes (IDE’s) have successfully been fabricated and characterized amperometrically for the electrochemical detection of the pH concentration and bio-molecules. In the present work, aluminium (Al) is used act as the metal contact (electrodes) deposited on the silicon substrate using a thermal evaporator (PVD) vacuum coater. The simple conventional photolithography was applied to fabricate the micro-gap capacitive transducer. IDE’s mask is designed using the aid of AutoCAD software for the detection of bio-substance which is an extremely small scale in size before being transferred to commercial chrome mask. The fabricated micro-gap sensor will then further evaluated through surface topology and electrical characteristic. The surface topology was implemented using AFM, LPM, SEM and HPM. The electrical measurements were carried out using a Dielectric analyzer (DA) with 2400 source meter (KEITHLEY) to examine the electrical characteristics such as capacitance and conductance. Purchased pH buffer solutions which varied from pH4 to pH 10 is dropped on the surface of microelectrode and the effect on it is investigated for the application in pH measurement. This study has proven that an increase in the concentration of acidic to alkaline pH leads to proportional to the capacitance.
Corresponding Author: Uda Hashim
Corresponding Author’s Email: uda@unimap.edu.my
Full text: PDF
Fluorescence-Signaling Aptasensor for ATP and PDGF Detection on Functionalized Diamond Surface
Abstract – An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 nMand 0.2 μMof PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding.
Corresponding Author: Ruslinda Abdul Rahim
Corresponding Author’s Email: ruslinda@unimap.edu.my
Full text: PDF
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