Institute of Nano Electronic Engineering

Universiti Malaysia Perlis

  • Facebook
  • Instagram
  • Twitter
  • YouTube
  • About Us
    • Background
    • Mission and Vision
    • Organization
    • Client’s Charter
    • Corporate Video
    • Our Expertise
    • Acknowledgement
  • Research
    • Research Facilities
    • Research Activities
      • Research Focus
      • Research Projects
        • Products
      • Postgraduate
        • Current Students
        • Alumni
      • Collaborations
  • Publications
    • Scoreboard
  • Contact Us
    • Staff Directory
    • Feedback
  • Gallery
    • Video
    • Audio
  • Download
    • Archive
    • Publications
    • Forms
    • OBE Awareness
    • Reports
  • Quick Links
    • Support Links
    • FAQ
    • Sitemap
    • Faculty
    • Other Departments
    • Events
You are here: Home / Publications / Nanoparticle sensor for label free detection of swine DNA in mixed biological samples

Nanoparticle sensor for label free detection of swine DNA in mixed biological samples

April 1, 2014 By Editor

Abstract – We used 40 ± 5 nm gold nanoparticles (GNPs) as colorimetric sensor to visually detect swine-specific conserved sequence and nucleotide mismatch in PCR-amplified and non-amplified mitochondrial DNA mixtures to authenticate species. Colloidal GNPs changed color from pinkish-red to gray-purple in 2 mM PBS. Visually observed results were clearly reflected by the dramatic reduction of surface plasmon resonance peak at 530 nm and the appearance of new features in the 620–800 nm regions in their absorption spectra. The particles were stabilized against salt-induced aggregation upon the adsorption of single-stranded DNA. The PCR products, without any additional processing, were hybridized with a 17-base probe prior to exposure to GNPs. At a critical annealing temperature (55 ◦C) that differentiated matched and mismatched base pairing, the probe was hybridized to pig PCR product and dehybridized from the deer product. The dehybridized probe stuck to GNPs to prevent them from salt-induced aggregation and retained their characteristic red color. Hybridization of a 27-nucleotide probe to swine mitochondrial DNA identified them in pork–venison, pork–shad and venison–shad binary admixtures, eliminating the need of PCR amplification. Thus the assay was applied to authenticate species both in PCR-amplified and non-amplified heterogeneous biological samples. The results were determined visually and validated by absorption spectroscopy. The entire assay (hybridization plus visual detection) was performed in less than 10 min. The LOD (for genomic DNA) of the assay was 6 μg ml−1 swine DNA in mixed meat samples. We believe the assay can be applied for species assignment in food analysis, mismatch detection in genetic screening and homology studies between closely related species.

Keywords – nanoparticles, dna, biological

Corresponding Author: Uda Hashim
Corresponding Author’s Email: uda@unimap.edu.my

Full text: PDF

Share this:

  • Click to share on Facebook (Opens in new window)
  • Click to share on Twitter (Opens in new window)
  • Click to share on Pinterest (Opens in new window)
  • Click to email a link to a friend (Opens in new window)
  • More
  • Click to share on Reddit (Opens in new window)
  • Click to share on LinkedIn (Opens in new window)
  • Click to share on WhatsApp (Opens in new window)
  • Click to print (Opens in new window)
  • Click to share on Telegram (Opens in new window)

Filed Under: Publications Tagged With: biological, dna, nanoparticles

NEWS @ INEE

  • Postgraduate Studies Opportunity
  • Congratulations Prof. Ir. Dr. Mohd Khairuddin Md Arshad
  • Assoc. Prof. Ts. Dr. Muhammad Mahyiddin Ramli Appointed as New Director of INEE
  • Assoc. Prof. Ts. Dr. Foo Kai Loong Appointed as New Deputy Director of INEE
  • Dr. Mohamad Faris Mohamad Fathil Appointed as New Postgraduate Program Chairman of INEE

EVENTS & ACTIVITIES

  • Call for Paper IEEE International Conference on Sensor & Nanotechnology (SenNano2023)
  • Call for Papers 2022 Joint International Conference on Nanoscience & Nanoengineering (BOND 21 2022)
  • Trace Analysis and Biosensor International Symposium I: Emerging Challenges and Opportunities
  • CSR & STEM Programme: Exploration in The Nano World
  • Nanotechnology and Biosensor Colloquium 2019
Privacy Policy | Security Policy | Disclaimer | Sitemap | FAQ | Feedback | User Guide | Download | Postgraduate

Institute of Nano Electronic Engineering, Universiti Malaysia Perlis
Lot 106, 108 & 110, Blok A, Taman Pertiwi Indah,
Jalan Kangar-Alor Setar, Seriab 01000 Kangar, Perlis, Malaysia
Tel: +604-979 8581 Fax: +604-979 8578 Email: webmaster.inee@unimap.edu.my

Copyright © INEE UniMAP

  • Facebook
  • Instagram
  • Twitter
  • YouTube

For best view, please use latest version of Internet Explorer, Mozilla Firefox and Google Chrome with resolution 1280 x 768 and above.

QS 5 Stars

MQA

MSC Malaysia

myGov

Logo UniMAP

Visits 302 Total 190990

Designed by Mohd Isa from Portal Kerjaya & Sumber Informasi Malaysia