Institute of Nano Electronic EngineeringTemp files

Universiti Malaysia Perlis

  • Facebook
  • Twitter
  • YouTube
  • About Us
    • Background
    • Mission and Vision
    • Organization
    • Client’s Charter
    • Corporate Video
    • Our Expertise
    • Acknowledgement
  • Research
    • Research Facilities
    • Research Activities
      • Research Focus
      • Research Projects
        • Products
      • Postgraduate
        • Current Students
        • Alumni
      • Collaborations
  • Publications
    • Scoreboard
  • Contact Us
    • Staff Directory
    • Feedback
  • Gallery
    • Video
    • Audio
  • Download
    • Archive
    • Publications
    • Forms
    • OBE Awareness
    • Reports
  • Quick Links
    • Support Links
    • FAQ
    • Sitemap
    • Schools & Faculty
    • Other Departments
You are here: Home / Publications / Production and purification of antibody by immunizing rabbit with rice tungro bacilliform and rice tungro spherical viruses

Production and purification of antibody by immunizing rabbit with rice tungro bacilliform and rice tungro spherical viruses

May 10, 2019 By Editor

Abstract – Rice tungro disease is the major disease caused by infection with the rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). In this study, New Zealand White rabbits were immunized with pure viruses for the production of antibodies against both species. The production of polyclonal antibodies against Tungro viral disease using ammonium sulfate precipitation and a protein A affinity column and their assessment are described. Two peaks were found from the protein A affinity column. Peak 1 represents the unbound compounds from the extracted serum and peak 2 represents antibody that bound to protein A, which was eluted using elution buffer. Peak 2 was collected for antibody titration. The amount of pure antibody in the titers was quantified by enzyme-linked immunosorbent assay (ELISA) to capture the tungro viruses. Antibody titer was analyzed by the ELISA method. For anti-RTBV, 1.696 mg/mL was highest at the second bleed and anti-RTSV was 2.3225 mg/mL was highest at the first bleed. These antibodies detected the tungro viral disease well and proved to be a potential probe for the detection of rice tungro disease.

Corresponding Author: Nor Azizah Parmin
Corresponding Author’s Email: norazizahparmin84@gmail.com

Download: PDF

Share this:

  • Click to share on Facebook (Opens in new window)
  • Click to share on Twitter (Opens in new window)
  • Click to share on Pinterest (Opens in new window)
  • Click to email this to a friend (Opens in new window)
  • More
  • Click to share on Reddit (Opens in new window)
  • Click to share on LinkedIn (Opens in new window)
  • Click to share on WhatsApp (Opens in new window)
  • Click to print (Opens in new window)
  • Click to share on Telegram (Opens in new window)

Filed Under: Publications

RSS NEWS @ INEE

  • INEE Wins 100% Medal at EREKA 2021
  • INEE Researcher Awarded FRGS 2020 Grant
  • Congratulations to Assoc. Prof. Ir. Dr. Mohd Khairuddin and team for a publication in Current Medicinal Chemistry
  • INEE Postgraduate Supervision 2020
  • Postgraduate Program Offered

RSS EVENTS & ACTIVITIES

  • Trace Analysis and Biosensor International Symposium I: Emerging Challenges and Opportunities
  • CSR & STEM Programme: Exploration in The Nano World
  • Nanotechnology and Biosensor Colloquium 2019
  • Transformation of Smart Sensor Technology in Real World
  • Research Talk by Dr. Joe Welch from UCL
Privacy Policy | Security Policy | Disclaimer | Sitemap | FAQ | Feedback | User Guide | Download | Postgraduate

Institute of Nano Electronic Engineering, Universiti Malaysia Perlis
Lot 106, 108 & 110, Blok A, Taman Pertiwi Indah,
Jalan Kangar-Alor Setar, Seriab 01000 Kangar, Perlis, Malaysia
Tel: +604-979 8581 Fax: +604-979 8578 Email: webmaster.inee@unimap.edu.my

Copyright © INEE UniMAP

For best view, please use latest version of Internet Explorer, Mozilla Firefox and Google Chrome with resolution 1280 x 768 and above.

QS 5 Stars

MQA

MSC Malaysia

myGov

UniMAP




Designed by Mohd Isa from Portal Kerjaya & Sumber Informasi Malaysia

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.