Institute of Nano Electronic Engineering

Universiti Malaysia Perlis

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Monitoring recombinant human erythropoietin abuse among athletes

September 30, 2015 By Editor

Abstract – The illegal administration of recombinant human erythropoietin (rHuEPO) among athletes is largely preferred over blood doping to enhance stamina. The advent of recombinant DNA technology allowed the expression of EPO-encoding genes in several eukaryotic hosts to produce rHuEPO, and today these performance-enhancing drugs are readily available. As a mimetic of endogenous EPO (eEPO), rHuEPO augments the oxygen carrying capacity of blood. Thus, monitoring the illicit use of rHuEPO among athletes is crucial in ensuring an even playing field and maintaining the welfare of athletes. A number of rHuEPO detection methods currently exist, including measurement of hematologic parameters, gene-based detection methods, glycomics, use of peptide markers, electrophoresis, isoelectric focusing (IEF)-double immunoblotting, aptamer/antibody-based methods, and lateral flow tests. This review gleans these different strategies and highlights the leading molecular recognition elements that have potential roles in rHuEPO doping detection.

Corresponding Author: Subash C B Gopinath
Corresponding Author’s Email: subash@unimap.edu.my

Full text: PDF

Filed Under: Publications Tagged With: Antibody, Aptamer, Athletes, Doping, RHuEPO

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