Publications Archives – Page 3 of 12 – Institute of Nano Electronic Engineering

Production and purification of antibody by immunizing rabbit with rice tungro bacilliform and rice tungro spherical viruses

May 10, 2019 By Editor

Abstract – Rice tungro disease is the major disease caused by infection with the rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). In this study, New Zealand White rabbits were immunized with pure viruses for the production of antibodies against both species. The production of polyclonal antibodies against Tungro viral disease using ammonium sulfate precipitation and a protein A affinity column and their assessment are described. Two peaks were found from the protein A affinity column. Peak 1 represents the unbound compounds from the extracted serum and peak 2 represents antibody that bound to protein A, which was eluted using elution buffer. Peak 2 was collected for antibody titration. The amount of pure antibody in the titers was quantified by enzyme-linked immunosorbent assay (ELISA) to capture the tungro viruses. Antibody titer was analyzed by the ELISA method. For anti-RTBV, 1.696 mg/mL was highest at the second bleed and anti-RTSV was 2.3225 mg/mL was highest at the first bleed. These antibodies detected the tungro viral disease well and proved to be a potential probe for the detection of rice tungro disease.

Corresponding Author: Nor Azizah Parmin
Corresponding Author’s Email: norazizahparmin84@gmail.com

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The ZnO-FET Biosensor for Cardiac Troponin I

July 23, 2018 By Editor

Abstract – This paper investigates the influence of substrate-gate coupling on the ZnO-FET biosensor’s sensitivity for detection of cardiac troponin I (cTnI), a ‘gold standard’ biomarker for acute myocardial infarction (AMI). The FET-based device with introduction of substrate-gate coupling on p-type silicon-on-insulator (SOI) substrate is fabricated using conventional lithography processes. An n-type zinc oxide (ZnO) thin film deposited via electron-beam evaporator is used as transducer for bridging the source and drain regions. Surface modifications via functionalization with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA) as chemical linkers, followed by immobilization of cTnI monoclonal antibody (MAb-cTnI) as bio-receptor on the ZnO thin film allow different concentration of cTnI detection with high selectivity. The device’s sensitivity increases up to 9 %•(g/ml)-1 with the increase of the substrate-gate voltage (VSG) up to -10 V at very low limit of detection (LOD) down to 1.6 fg/ml.

Corresponding Author: Mohamad Faris Mohamad Fathil
Corresponding Author’s Email: mohamadfaris@unimap.edu.my

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Eco-friendly synthesis of Solanum trilobatum extract-capped silver nanoparticles is compatible with good antimicrobial activities

March 19, 2018 By Editor

Abstract – This study focused on the evaluation of antimicrobial activity of silver nanoparticles (AgNPs) after their green synthesis by means of a Solanum trilobatum bark extract. The obtained product with an intense surface plasmon resonance band at ∼442 nm with UV–visible spectroscopic analysis indicated the formation of AgNPs. The morphology of AgNPs was observed under transmission electron microscopy and field emission scanning electron microscopy, displayed that the eco-friendly synthesized AgNPs have a spherical shape with an average size of ∼25 nm in diameter. X-ray powder diffraction and selected area electron diffraction analyses confirmed that the AgNPs are crystalline in nature. Fourier transform infrared spectroscopy indicated that the AgNPs capped with active ingredients of the bark extract. X-ray photoelectron spectroscopy revealed elemental composition of the AgNPs. The performance of S. trilobatum bark extract-capped AgNPs in terms of inhibition of microbial growth was studied by disc diffusion and well diffusion assays. Eco-friendly synthesized S. trilobatum extract-capped AgNPs were found to possess enhanced antimicrobial properties: growth inhibition of gram-negative and gram-positive bacteria and of fungal species. These results demonstrated the potential applications of the indigenous medicinal plants to the field of nanotechnology.

Corresponding Author: Subash C B Gopinath
Corresponding Author’s Email: subash@unimap.edu.my

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Reduced graphene oxide–multiwalled carbon nanotubes composites as sensing membrane electrodes for DNA detection

December 12, 2017 By Editor

Abstract – Thin films of reduced graphene oxide–multiwalled carbon nanotube (rGO–MWCNT) composites were demonstrated as sensing membrane electrodes for single-stranded DNA (ssDNA) detection. The morphology of the rGO–MWCNT composite thin films was observed via field emission scanning electron microscopy. The GO sheet and MWCNTs were clearly obtained, and the MWCNTs were uniformly distributed on the surface of the GO. The chemical bonding of the rGO–MWCNTs was examined using Fourier transform infrared spectroscopy. The element compositions of carbon, silicon, and oxygen were confirmed via energy-dispersive X-ray spectroscopy and X-ray powder diffraction analysis. The biosensor demonstrated high sensitivity to the ssDNA target with a linear range from 500 to 100 pM. Furthermore, the biosensor demonstrated good selectivity, reproducibility, and long-term stability for DNA detection. Since, the biosensor responded very-well and demonstrated excellent detection capabilities, it is highly recommended to be used in detecting specific biomarkers and other targeted proteins.

Corresponding Author: Ruslinda A. Rahim
Corresponding Author’s Email: ruslinda@unimap.edu.my

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Selectivity verification of cardiac troponin monoclonal antibodies for cardiac troponin detection by using conventional ELISA

July 16, 2017 By Editor

Abstract – This paper presents preparation and characterization of conventional enzyme-linked immunosorbent assay (ELISA) for cardiac troponin detection to determine the selectivity of the cardiac troponin monoclonal antibodies. Monoclonal antibodies, used to capture and bind the targets in this experiment, are cTnI monoclonal antibody (MAb-cTnI) and cTnT monoclonal antibody (MAb-cTnT), while both cardiac troponin I (cTnI) and T (cTnT) are used as targets. ELISA is performed inside two microtiter plates for MAb-cTnI and MAb-cTnT. For each plate, monoclonal antibodies are tested by various concentrations of cTnI and cTnT ranging from 0-6400 μg/l. The binding selectivity and level of detection between monoclonal antibodies and antigen are determined through visual observation based on the color change inside each well on the plate. ELISA reader is further used to quantitatively measured the optical density of the color changes, thus produced more accurate reading. The results from this experiment are utilized to justify the use of these monoclonal antibodies as bio-receptors for cardiac troponin detection by using field-effect transistor (FET)-based biosensors coupled with substrate-gate in the future.

Corresponding Author: Ruslinda A. Rahim
Corresponding Author’s Email: ruslinda@unimap.edu.my

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